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Detectives from the Metropolitan Police's Flying Squad have taken over the investigation into a robbery at the Dean Street Townhouse in Soho.
Officers were called to the hotel in Dean Street, shortly after 03.05hrs on Tuesday, 26 August, following a report that two masked men, armed with long-barrelled weapons, had entered the office area and demanded money from two male employees.
The members of staff were threatened, and at this early stage in the investigation it is believed at least one of them was tied up.
Both suspects then left the hotel with an amount of cash and mobile phones.
No one was injured during the incident, however, one of the victims was treated for shock.
No shots were fired.
The suspects have been described as speaking with east European accents and were both wearing white hoodies. No further descriptions at this stage.
Detective Inspector Spencer Barnett, Flying Squad, said: "This was a terrifying experience for the two employees, who were simply going about their usual duties.
"At this early stage in the investigation we believe this was a commercial robbery with the target being the office, rather than guests staying at the hotel and we do not believe the suspects went beyond this staff only area.
"We are working around the clock to secure CCTV and forensic opportunities and our investigation is moving at a fast pace.
"I would like to take this opportunity to appeal for any witnesses who have not yet come forward or anyone who has information about this crime to contact us in confidence."
Anyone who witnessed the incident, was in the area at the time or has information that could assist officers is asked to call 101 or to remain anonymous, call Crimestoppers on 0800 555 111.